Research Article

Acetate Activation in Methanosaeta thermophila: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase

Table 1

Gene number, function of corresponding protein and primers used for amplification of genes analyzed by qRT-PCR.

FunctionGene numberPrimer sequence

AMP-dependent ACSmthe_1194for CCAGTGGATCATCGAGTA
rev CAGAAATCGAGGTAGTTC
mthe_1195for TAAGGAGCTTGCTGAGAA
rev CAGAACTCTATGTAGTGG
mthe_1196for TCGAAGGCGTATGCTGAC
rev CGCCTCGTCAGCCTGCTT
mthe_1413for CAGGCGCGCTCCGCGAG
rev GGCCTTTATCGGGATAGG

ADP-dependent ACSmthe_0554for TATCATTGGGGTTACAAG
rev CAGAGATGGGTATTGATC

PPiasemthe_0236for GCCAGCATGTATGAGCTG
rev CATGTGGGTGACTTGAAT

GAP-DHmthe_0701for CTATGCCGTTGCTGTGAA
rev TTGGCGGTGCATTTATCT

ribosomal protein S3Pmthe_1722for GTTCGTCATGATTGGCAC
rev CCCCTTCTGGAGCTTATC

intergenic regionBetween mthe_1194for GCGGTCAACCTATTTTATTT
and mthe_1195rev TTACATACCTCCATTCATCT

intergenic regionBetween mthe_1195for AACGTCCGCAATTTTTATTT
and mthe_1196rev CTGCCTCCAGCCCATCCCG