Archaeal Phospholipid Biosynthetic Pathway Reconstructed in Escherichia coli
Figure 2
In vitro assay of the archaeal enzymes for phospholipid biosynthesis. (a) Thin-layer radiochromatogram of the dephosphorylated products from the reactions with recombinant M. acetivorans GGPP synthase, GGGP synthase, and/or DGGGP synthase. The enzyme assays were performed using FPP, [14C]IPP, and α-glycerophosphate as the substrates. (b) Thin-layer radiochromatogram of the products from the reaction with recombinant M. acetivorans G-1-P dehydrogenase, coupled with GGPP synthase and GGGP synthase. FPP, [14C]IPP, and DHAP were used as the substrates. S.F., solvent front; Ori., origin.
