Role of Motif III in Catalysis by Acetyl-CoA Synthetase

Figure 2

Inhibition of Acs1_Mt. (a) Ribose, (b) adenosine, (c) 2′-deoxyadenosine, and (d) 3′-deoxyadenosine. Assays were performed with the indicated concentrations of inhibitor in the reaction, and results are plotted as a percentage of the activity observed in the absence of inhibitor, with error bars as shown. The 𝐾_𝑖 value for each inhibitor was determined by extrapolation of the data.

(a)

(b)

(c)

(d)