DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii

Figure 1

(a) Schematic representation of the arrangement of OB-folds (light grey) and CCCH zinc fingers (dark grey) and respective amino acid positions in HvRPA3 and MacRPA3 [10]. Constructs used in this study are indicated. (b) SDS-PAGE analysis of 2.5 μg purified full length HvRPA3. The 6xHis-tagged construct has a predicted MW of 35.4×10³. (c) Size exclusion profiles of HvRPA3 in 0.2 M (solid line), 1 M (dashed line), and 3 M (dotted line) KCl. The 𝑥 axis shows elution volume (mLs) and the 𝑦 axis absorbance at 280 nm.

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