DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii
Figure 3
(a) Intrinsic fluorescence spectra for HvRPA3 incubated in 0.2 (dotted line), 1 M (dashed line), and 3 M (solid line) KCl. The axis shows the wavelength of the emission spectra (nm) and the axis the observed fluorescence. (b) Homology modelling of the HvRPA3 OB-fold (76–170). The HvRPA3 model (dark grey) and human RPA70-ssDNA (1JMC) complex (light grey) are shown in cartoon representation in two orthogonal views [27]. DNA is shown in stick form. The two tryptophan residues in HvRPA3 are shown in stick representation. 1JMC contains two OB-folds and a 8mer ssDNA, for clarity only the first OB-fold and the proximal 4 nucleotides are shown. Produced using PyMol [34].
