DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile <i>Haloferax volcanii</i>

<table>(a) SDS-PAGE analysis of 2.5 <i>μ</i>g purified HvRPA3-NTD, HvRPA3-CTD-1, and HvRPA3-CTD-2. The 6xHis-tagged constructs have predicted MWs of <svg height="14.375" id="M40" style="vertical-align:-0.3135pt" version="1.1" viewbox="0 0 66.150002 14.375" width="66.150002" xmlns="http://www.w3.org/2000/svg">
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</svg> (HvRPA3-NTD) and <svg height="14.375" id="M41" style="vertical-align:-0.3135pt" version="1.1" viewbox="0 0 66.150002 14.375" width="66.150002" xmlns="http://www.w3.org/2000/svg">
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</svg> (HvRPA3-CTD). (b) Predicted MWs (<svg height="9.8625002" id="M42" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 7.875 9.8625002" width="7.875" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="8.0019197" y="0">1</tspan>
<tspan style="font-size: 12.50px; " x="14.25342" y="0">0</tspan>
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</svg>) from size exclusion chromatography analysis of HvRPA3-NTD, HvRPA3-CTD-1, and HvRPA3-CTD-2 in 0.2, 1, and 3 M KCl (light, mid, and dark grey, resp.). HvRPA3-NTD in complex with 18mer ssDNA is also shown.</table>

Archaea

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Figure 4

Figure 4: DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile <i>Haloferax volcanii</i> 

Archaea

DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii

Figure 4