DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii
Figure 6
(a) Comparison showing the improved fit of a two-site binding model (dotted line) versus the Hill binding model employed for analysis (dashed line) for the HvRPA3-NTD dimer in 1 M KCl. The axis displays the concentration of the dimer with the y axis indicating normalised FA. (b) Comparison of binding of dimeric HvRPA3-NTD in 1 M (solid line) fitted to the two-site model displayed in (a) and the monomeric form in 3 M (dashed line) KCl fitted to a Hill binding model. The axis displays the concentration of the proteins with the y axis indicating normalised FA. (c) Intrinsic fluorescence spectra for HvRPA3-NTD incubated in 0.2 (dotted line), 1 M (dashed line), and 3 M (solid line) KCl. The axis shows the wavelength of the emission spectra (nm) and the axis the observed fluorescence.
