DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii

Table 1

Dissociation constants and Hill coefficients calculated from a Hill binding model for HvRPA3 constructs compared with dimeric MacRPA3 [10]. A two-site binding model was employed for the NTD analysis in 1 M KCl as described in Section 2. Protein concentration is for the monomer equivalent, with the exception of NTD in 1 M KCl, where dimeric concentration was employed in model fitting.

[KCl]	𝐾𝑑 (nM)	Hill coefficient
Full length 1 M	53.9±18.3	0.9±0.1
Full length 3 M	24.1±2.9	1.2±0.1
MacRPA3	5.92 ± 0.23	1.46 ± 0.08
NTD 1 M
High	4.1±7.9
Low	246.6±195.5
NTD 3 M	212.5±67.6	1.1±0.2