Research Article

DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii

Table 2

ICP-MS analysis of zinc binding. Values shown are averages of triplicate samples of 250 μg of the indicated protein in 500 μL volume.

Protein Mean Zn (μg/L)μM ZnZn/mol

Full length 5 5 7 . 8 ± 4 . 8 8.530.60
NTD 1 0 4 . 0 ± 5 . 2 1.590.06
CTD-1 4 5 . 1 ± 2 . 1 0.690.03
CTD-2 7 8 7 . 1 ± 1 6 . 1 12.030.53