Crystal Structure of a 9-Subunit Archaeal Exosome in Pre-Catalytic States of the Phosphorolytic Reaction
Figure 2
High-resolution view of the inorganic phosphate binding site. (a) Unbiased Fo-Fc map at 3 sigma is shown in magenta and 2Fo-Fc map at 1 sigma in blue. The inorganic phosphate and contacting residues from the Rrp41 subunit are shown as sticks. (b) Schematic representation of the inorganic phosphate binding site with distances between the Pi ion and protein residues indicated. (c) Structures of the S. solfataricus and the M. thermautotrophicus exosomes superimposed reveal that the Pi-binding sites are very well conserved across different archaeal species.
