Low Rates of Lateral Gene Transfer among Metabolic Genes Define the Evolving Biogeochemical Niches of Archaea through Deep Time

Table 3

Nitrogen metabolism.

Gene name	Gene	Linked to	Number of LGT events	Monophyletic clades
Nitrate reductase, alpha subunit	NarG	NarH, NorB in Pyrobaculum spp.	7	(1) Ferroglobus; (2) Haloarcula, Haloferax, Halogeometricum, Halomicrobium, Halorhabdus, and Halorubrum; (3) Aeropyrum; (4) P. aerophilum, P. arsenaticum, and P. Calidifontis; (5) S. islandicus M.14.25, S. islandicus M.16.27; (6) Metallosphaera yellowstonensis; (7) Vulcanisaeta distributa
Nitrate reductase, beta subunit	NarH	NarG, NorB in Pyrobaculum spp.	(7)	(1) Ferroglobus; (2) Haloarcula, Haloferax, Halogeometricum, Halomicrobium, Halorhabdus, and Halorubrum; (3) Aeropyrum; (4) P. aerophilum, P. arsenaticum, and P. Calidifontis; (5) S. islandicus M.14.25, S. islandicus M.16.27; (6) Metallosphaera yellowstonensis; (7) Vulcanisaeta distributa
Nitrite reductase, Cu-containing	NirK		1 [2?]	(1) Haloarcula, Halobiforma, Haloferax, Halogeometricum, Halomicrobium, Halopiger, Halorhabdus, Haloterrigena, Natrinema, and Natronomonas
Nitrite reductase, Heme-containing	NirS		1	(1) Pyrobaculum aerophilum, P. arsenaticum, and P. calidifontis
Nitric oxide reductase	NorB	NarGH in Pyrobaculum spp.	4	(1) Haladaptatus, Haloarcula, Halobiforma, Haloferax, Halogeometricum, Halomicrobium, Halopiger, Halorubrum, Haloterrigena, and Natrinema; (2) Caldivirga, P. spp., Vulcanisaeta distributa, and Thermoproteus uzoniensis; (3) Acidilobus; (4) S. islandicus spp., S. solfataricus spp.
Nitrous oxide reductase	NosZ		3	(1) Haloarcula, Halobacterium, Halobiforma, Haloferax, Halogeometricum, Halopiger, and Halorubrum; (2) Ferroglobus; (3) P. calidifontis, P. sp. 1860
Nitrogenase reductase	NifH	NifD and NifE in Methanococcales, Methanobacteriales, Methanomicrobiales, and Methanosarcina	4	(1) Methanococcales (cluster II); (2) Methanobacteriales (cluster II); (3) Methanomicrobiales Methanosarcinales (cluster II); (4) Methanosarcina (cluster III)
Nitrogenase, alpha subunit	NifD	NifH and NifE in Methanococcales, Methanobacteriales, Methanomicrobiales, and Methanosarcina	(4)	(1) Methanococcales (cluster II); (2) Methanobacteriales (cluster II); (3) Methanomicrobiales Methanosarcinales (cluster II); (4) Methanosarcina (cluster III)
Nitrogenase Fe Mo cofactor	NifE	Nif H and NifD in Methanococcales, Methanobacteriales, Methanomicrobiales, and Methanosarcina	(4)	(1) Methanococcales (cluster II); (2) Methanobacteriales (cluster II); (3) Methanomicrobiales Methanosarcina (cluster II); (4) Methanosarcina (cluster III)