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Research Article

Impact of Trichloroethylene Exposure on the Microbial Diversity and Protein Expression in Anaerobic Granular Biomass at 37°C and 15°C

Table 2

Proteins identified from bioreactor biomass. Ratio = TCE reactor spot intensity: non-TCE control reactor spot intensity, that is, positive values indicate the protein in question was expressed at a higher intensity in the TCE-supplemented reactor, and negative values indicate the protein was expressed at a higher intensity in the control reactor. N.D.: not detected.


Protein	Suggested function	Accession number	Species assignment	Classification	Mascot score	% coverage	37°C ratio	15°C ratio

Acetyl-CoA decarbonylase β-subunit	Methanogenesis from acetate	gi 116753609	Methanosaeta thermophila	Order Methanosarcinales	253	8	−1.7	−1.2
Acetyl-CoA decarbonylase β-subunit	Methanogenesis from acetate	gi 116753609	Methanosaeta thermophila	Order Methanosarcinales	171	8	N.D.	1.2
Co-enzyme F420 dependent N5, N10 methylenetetrahydromethanopterin reductase	Methanogenesis from CO₂	gi 1002717	Methanothermobacter thermoautrophicus	Order Methanobacteriales	248	19	−1.4	−1.7
					166	13	−2.6	N.D.
					147	9	1.8	1
Tetrahydromethanopterin-S-methyltransferase H-subunit	Methanogenesis from CO₂	gi 116754675	Methanosaeta thermophila	Order Methanosarcinales	104	2	−3.9	N.D.
Methyl-CoM reductase I α-subunit	Methanogenesis	gi 126855	Methanothermobacter marburgensis	Order Methanobacteriales	203	8	3.2	−1.6
Methyl-CoM reductase I α-subunit	Methanogenesis	gi 284413635	Methanobacterium sp.	Order Methanobacteriales	469	25	1.2	−1.9
					396	23	N.D.	−4.6
					237	16	N.D.	−3.7
Methyl-CoM reductase I α-subunit	Methanogenesis	gi 47827047	Uncultured Methanosarcinales	Order Methanosarcinales	140	17	2.1	2.8
Methyl-CoM reductase I β-subunit	Methanogenesis	gi 126862	Methanothermobacter marburgensis	Order Methanobacteriales	393	11	N.D.	−8.3
					223	8	−2.7	N.D.
					148	9	−1.7	−1.5
Methyl-CoM reductase I β-subunit	Methanogenesis	gi 126178567	Methanoculleus marisnigri	Order Methanomicrobiales	224	5	2.8	1.3
Methyl-CoM reductase I γ-subunit	Methanogenesis	gi 116753883	Methanosaeta thermophila	Order Methanosarcinales	157	11	−2.7	4.9
Proteasome α-subunit	Proteolysis	gi 6093782	Methanosaeta thermophila	Order Methanosarcinales	206	16	−5.1	12.5
Proteasome α-subunit	Proteolysis	gi 6093782	Methanosaeta thermophila	Order Methanosarcinales	151	12	N.D.	1.1
Thermosome	Molecular chaperone	gi 116754081	Methanosaeta thermophila	Order Methanosarcinales	272	9	−2.9	N.D. in R4
Thermosome	Molecular chaperone	gi 116754081	Methanosaeta thermophila	Order Methanosarcinales	273	10	N.D.	1.8
V-type ATP synthase α-subunit	ATP synthesis	gi 116754898	Methanosaeta thermophila	Order Methanosarcinales	217	10	−1.1	N.D.
					212	10	3.9	N.D.
					199	10	1.9	N.D.
					191	8	−5.6	1.9
Hypothetical protein Mhun-2513	Unknown	gi 88603754	Methanospirillum hungatei	Order Methanomicrobiales	1640	46	1	N.D.
Acetate-CoA Ligase	Acetyl-CoA synthesis	gi 116754497	Methanosaeta thermophila	Order Methanosarcinales	153	5	3.8	5.9
Iron-containing alcohol dehydrogenase	Ethanol metabolism	gi 77457538	Pseudomonas fluorescens	Phylum Proteobacteria	180	3	1.4	N.D.
					159	3	1	−1.7
					122	6	N.D.	1.1
Phosphate acetyltransferase	Acetate metabolism	gi 95930364	Desulfuromonas acetoxidans	Phylum Proteobacteria	106	5	−1.4	6.5
Acetate-CoA transferase β-subunit	Acetate metabolism	gi 147678377	Pelotomaculum thermopropionicum	Phylum Firmicutes	304	18	1.1	N.D.
Glyceraldehyde-3-phosphate dehydrogenase type I	Glycolysis	gi 213959469	Propionibacterium acnes	Phylum Actinobacteria	375	17	−2	2
Methyl Malonyl-CoA mutase large subunit	Isomerisation of Succinyl-CoA	gi 116747906	Syntrophobacter Fumaroxidans	Phylum Proteobacteria	507	16	23.7	−1.1
Succinyl-CoA synthetase β-subunit	Formation of Succinyl-CoA	gi 116749138	Syntrophobacter Fumaroxidans	Phylum Proteobacteria	235	15	−1.1	N.D.
Malate dehydrogenase	Glyoxylate pathway	gi 116747908	Syntrophobacter Fumaroxidans	Phylum Proteobacteria	383	26	1.8	−3.1
O-acetylserine sulfhydrylase	Homocysteine production	gi 148265277	Geobacter uraniireducens	Phylum Proteobacteria	216	10	2.9	3.4
Chaperone protein	Macromolecule assembly	gi 85859704	Syntrophus aciditrophicus	Phylum Proteobacteria	555	17	N.D.	2.8
Chaperone protein DnaK	Protein folding	gi 116748478	Syntrophobacter Fumaroidans	Phylum Proteobacteria	230	8	−1.5	N.D.
Chaperonin GroEL	Protein folding	gi 148266317	Geobacter uraniireducens	Phylum Proteobacteria	620	18	−8.5	3.6
Chaperonin GroEL	Protein folding	gi 148266317	Geobacter uraniireducens	Phylum Proteobacteria	326	10	3.6	N.D. in R4
Extracellular ligand binding receptor	Receptor activity	gi 116750526	Syntrophobacter Fumaroidans	Phylum Proteobacteria	671	28	−1.5	N.D.
Extracellular ligand binding receptor	Receptor activity	gi 116750526	Syntrophobacter Fumaroidans	Phylum Proteobacteria	309	17	1.2	−2.9