Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

Figure 3

Coupled MALDI-TOF/MS and HPTLC analyses of the total lipid extract of P. furiosus. The total lipid extract was applied on the TLC plate (200 μg) in two lanes; the lipid bands in one lane were charred after spraying with 5% sulphuric acid, while 9-aminoacridine was applied manually along the other lane obtaining a continuous deposition. The main bands on the TLC plate after charring are shown in the centre of the picture, while, on the right and the left of the TLC, are shown the negative spectra obtained by MALDI scanning of the lane covered with the matrix. Dashed lines on the TLC plate were used to mark pale lipid bands.