A Novel Inducible Protein Production System and Neomycin Resistance as Selection Marker for Methanosarcina mazei
Figure 1
Maps of the plasmids constructed during this study. (a) Plasmid map of pSM01 that is a pWM321 derivative containing in addition the p1687 promoter for inducible protein production. (b) Plasmid map of pWM321-neo that contains the resistance cassette (neo) for neomycin resistance instead of the pac gene. (c) pSM02 is a combination of pSM01 and pWM321-neo. It contains both the neomycin resistance cassette and the p1687 promoter for inducible protein production.
