The Effect of Saturated Fatty Acids on Methanogenesis and Cell Viability of Methanobrevibacter ruminantium

Table 1

Methane inhibition rate, K⁺ efflux, and cell viability in cell suspensions treated with C₁₂ in different concentrations in Experiment 2 (𝑛=3; means ± standard error).

Time	3 h			24 h
Treatment	K^+(mg/L)	CH4 inhibition (%)^1	Cell viability (%)^2	K^+(mg/L)	CH4 inhibition (%)^1	Cell viability (%)^2
Blank	12.8 ± 1.7^b	20.7 ± 8.1^b	75 ± 3^a	16.0 ± 0.2^b	13.5 ± 16.3^b	56 ± 2^ab
Control	11.1 ± 0.2^b	—^b	79 ± 2^a	16.3 ± 0.4^b	—^b	61 ± 5^a
10 μg/mL	12.8 ± 0.3^b	89.6 ± 5.1^a	24 ± 5^b	18.5 ± 0.1^a	95.1 ± 2.5^a	53 ± 7^ab
15 μg/mL	18.4 ± 0.2^a	99.8 ± 0.1^a	27 ± 4^b	19.3 ± 0.2^a	99.8± 0.1^a	35 ± 4^bc
30 μg/mL	19.0 ± 0.3^a	100.0 ± 0.1^a	29 ± 6^b	19.1 ± 0.4^a	100 ± 0.0^a	13 ± 3^c
P values	0.0003	<0.0001	<0.0001	<0.0001	0.0018	0.0004

a--cTreatment means with unequal superscripts are different at P < 0.05.
¹Calculated from methane production rate (μmol/mg cell DM/min) in percent of the value of the control group after 3 and 24 h, respectively.
²Percentage of live cells (green) of total cells (green, yellow, and red) as determined with the LIVE/DEAD BacLight Kit.