Archaea / 2013 / Article / Tab 4 / Research Article
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula Table 4 Relative activities of recombinant esterases in the presence of different chemical compounds. The effect of the additives was further tested for significance. Significant effects (
) are written in bold type.
Detergent Relative activity of PlpBW1 (%) Relative activity of EstBW1 (%) Relative activity of EstBW2 (%) AgNO3 106.80 ± 16.06 34.27 ± 1.66 163.17 ± 4.88 CaCl2 132.39 ± 9.63 85.35 ± 6.30 97.10 ± 0.98 CoCl2 131.17 ± 5.93 104.94 ± 7.29 64.00 ± 5.60 CTAB 274.39 ± 34.52 40.66 ± 6.67 8.11 ± 3.30 CuCl2 125.48 ± 2.70 81.03 ± 3.98 94.20 ± 0.57 EDTA 119.45 ± 7.50 101.47 ± 10.75 96.03 ± 4.24 FeCl3 106.67 ± 8.94 53.56 ± 12.56 99.47 ± 5.01 KCl 124.50 ± 19.51 99.29 ± 12.11 96.40 ± 5.55 2-Mercaptoethanol 84.82 ± 7.31 0.00 ± 0.00 78.74 ± 19.95 MgCl2 133.37 ± 7.25 90.38 ± 13.06 101.54 ± 2.87 MnCl2 72.07 ± 2.31 82.14 ± 14.16 48.90 ± 5.22 NaCl 131.24 ± 15.17 98.59 ± 20.07 92.42 ± 3.60 NiSO4 121.86 ± 14.71 89.64 ± 4.60 45.65 ± 8.03 PMSF 77.49 ± 16.91 0.08 ± 16.43 0 ± 4.16 SDS 68.45 ± 17.38 22.47 ± 9.64 39.49 ± 3.14 Tween 80 174.52 ± 2.29 60.70 ± 12.74 141.50 ± 4.94 ZnCl2 72.01 ± 1.77 59.26 ± 5.72 8.64 ± 1.71
Abbreviations: CTAB: cetyltrimethylammonium bromide; EDTA: ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulfate; PMSF: phenylmethylsulfonyl fluoride.