Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic Archaeon Sulfolobus metallicus
Table 2
Proteins upregulated in S. metallicus cells exposed to 100 mM Cu or 1 mM Cd.
Spot
Molecular weight (kDa)
Cu induction levels
Putative function
Microorganism related
Accesion number
1†
26.6
∞
Proteasome subunit
S. solfataricus
AAK41034
3†
70.1
∞
Ferredoxin oxidoreductase
S. solfataricus
AAK42926
4†
42.9
∞
Alcohol dehydrogenase
S. solfataricus
AAK43154
5
35
2.6
Hypothetical protein
S. acidocaldarius
YP_254976
6
27
∞
Hypothetical protein
P. aerophilum
NP_559889
9†
57
∞
Phosphoglycerate dehydrogenase
M. thermautotrophicus
AAB85466
13†
27
3.4
Unknown
—
—
14
28
∞
Unknown
—
—
15
35
5.5
Unknown
—
—
20†
46.1
∞
Glutamate dehydrogenase
S. solfataricus
AAK42230
21†
45
∞
Hypothetical protein
M. acetivorans
NP_618380.1
22
49
∞
Hypothetical protein
P. torridus
YP_022807
23†
51
∞
ATP synthase subunit B
S. solfataricus
AAK40880
24
58.5
∞
HSP60 subunit
S. shibatae
AAG37273.1
30†
48
∞
Unknown
—
—
31
16
ND
Unknown
—
—
32
14.4
ND
Putative transcription regulator
S. solfataricus
AAK40413
33
13.8
ND
Transcription factor nusA
S. solfataricus
AAK40563
Spots refer to those numbered in Figure 2. Proteins in Figure 2 which are not included in this table were not subjected to sequencing due to their low intensities in the gels. †Spot up-regulated in cells exposed to Cu and Cd. ND: not determined. Infinity symbol indicates that proteins were expressed only in presence of copper or cadmium. M. thermoautotrophicus: Methanobacterium thermoautotrophicus; M. acetivorans: Methanosarcina acetivorans; S. shibatae: Sulfolobus shibatae; P. torridus: Picrophilus torridus; P. aerophilum: Pyrobaculum aerophilum; S. acidocaldarius: Sulfolobus acidocaldarius.