Low-Temperature (10°C) Anaerobic Digestion of Dilute Dairy Wastewater in an EGSB Bioreactor: Microbial Community Structure, Population Dynamics, and Kinetics of Methanogenic Populations

Table 3

Maximum substrate utilising rate, apparent half-saturation constant, and maximum initial (utilising) velocity changes of granular sludge cultivated at 10°C in the EGSB during 335 days for acetate and H₂/CO₂.

	𝑇 (°C)	Substrate
		H2/CO2			Acetate
		𝐴max^a	𝐾𝑚^b	𝑉max^c	𝐴max^a	𝐾𝑚^b	𝑉max^c
Biomass day 0	37	1.230; (±0.148)	0.007; (±0.002)	0.580; (±0.046)	2.208; (±0.647)	0.088; (±0.032)	0.722; (±0.265)
	15	0.172; (±0.015)	0.020; (±0.004)	2.058; (±0.674)	0.419; (±0.103)	0.022; (±0.007)	0.265; (±0.125)
Biomass day 355	37	8.526; (±0.525)	0.558; (±0.419)	23.818; (±8.635)	1.638; (±0.746)	0.163; (±0.024)	2.422; (±0.336)
	10	0.199; (±0.071)	0.008; (±0.004)	1.276; (±0.004)	0.246; (±0.037)	0.006; (±0.003)	0.413; (±0.303)

aMaximum substrate utilising rate (g COD g VSS⁻¹ d⁻¹).
^bApparent half-saturation constant (g COD L⁻¹).
^cMaximum initial velocity (g COD L⁻¹ d⁻¹).