PH1: An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2

Table 3

Digestion of the PH1 genome by nucleases^a.

Nuclease (amount)	Proteinase K-treated		Untreated
	Control	PH1 DNA	Control	PH1 DNA
DNase I (RNase-free); (100 U)	+^b	+	+^b	+
Exonuclease III; (100 U)	+^b	+	+^b	+
Mung bean nuclease; (10 U)	+^c	−	+^c	−
Nuclease BAL-31; (1 U)	+^b	+	+^b	−
RNase A; (5 μg/mL)	+^d	−	+^d	−
T7 exonuclease; (10 U)	+^b	+	+^b	−

aExtracted nucleic acid from purified PH1 virus was treated with various nucleases and then analysed by gel electrophoresis to detect whether the nuclease digested (+) or did not digest (−) the genome. Control nucleic acids used to confirm activity of the nucleases were bλ DNA, ca DNA oligonucleotide, and dyeast transfer RNA.