PH1: An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2

Table 6

Transfection of haloarchaea by PH1 DNA.

Species^a	Efficiency of transfection/transformation with
	PH1 DNA^b (PFU/μg of DNA)	pUBP2 DNA^b,c (CFU/μg of DNA)
Har. hispanica	5.3 ± 0.5 × 10^3	1.4 ± 0.8 × 10^4
Har. marismortui	4.5 ± 0.7 × 10^3	1.8 ± 0.1 × 10^4
“Har. sinaiiensis”	3.2 ± 0.6 × 10^2	—
Halobacterium salinarum	—	5.4 ± 4.2 × 10^3
Haloferax gibbonsii	2.8 ± 0.8 × 10^3	4.0 ± 2.6 × 10^3
Haloferax lucentense	—	5.7 ± 1.1 × 10^3
Hfx. volcanii	—	2.1 ± 0.7 × 10^5
Hrr. lacusprofundi	7.0 ± 2.2 × 10^2	9.5 ± 1.5 × 10^3
Haloterrigena turkmenica	1.6 ± 1.7 × 10^2	2.4 ± 0.6 × 10^3
Natrialba asiatica	3.5 ± 3.9 × 10^2	3.1 ± 1.0 × 10^4

aOnly those species positive for transfection and/or plasmid transformation are shown.
bRates of transfection by (nonprotease treated) PH1 DNA or transformation by plasmid pUB2 are averages of three independent experiments, each performed in duplicate (± standard deviation).
cTransformants were selected on plates with 2, 4, or 6 μg/mL simvastatin, depending on the strain.
— no plaques or colonies observed.