Research Article
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Table 2
In vitro evaluation of primer pairs by real-time PCR. DNA extracted from archaeal owners of the target gene served as positive control template during the optimization of annealing temperature () and extension time (
ext).Expected lengths of the amplification product refer to distances between primer binding sites in the archaeal sequences that were used during primer design. Observed product lengths were determined by agarose gel electrophoresis of the actual PCR products.
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