Comparative Analysis of Barophily-Related Amino Acid Content in Protein Domains of Pyrococcus abyssi and Pyrococcus furiosus

Figure 3

Mean relative amino acid abundance (MAA) analysis of the set of entire polypeptide sequences. (a) On average, both Pyrococci seem to use barophilic and nonbarophilic amino acids to the same extent. (b) The barophilic Asp, Val, and Arg are used significantly more in sequences of the barophilic P. abyssi compared to the nonbarophilic P. furiosus (positive difference), whereas the nonbarophilic Tyr, Gln, Thr, Ile, Lys, and Asn are used significantly more in P. furiosus compared to P. abyssi (negative difference). Statistical significance is marked with stars according to Welch’s two-sided test: ^∗𝑃<0.1; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001; ^∗∗∗∗𝑃<0.0001. A total of 1,896 P. abyssi and 2,125 P. furiosus sequences were analyzed.

(a)

(b)