920241.fig.003a
(a)
920241.fig.003b
(b)
920241.fig.003c
(c)
920241.fig.003d
(d)
920241.fig.003e
(e)
920241.fig.003f
(f)
920241.fig.003g
(g)
920241.fig.003h
(h)
920241.fig.003i
(i)
920241.fig.003j
(j)
Figure 3: Localization of MCR in Methanosarcina mazei (DSM 3647; a, b, c), Methanothermobacter marburgensis (d–g), and Methanothermobacter wolfei (h, i, j). On media without nickel depletion, the markers are localized in the cytoplasm (b, e, i). On nickel-depleted media with levulinic acid (c, f: 0.05 M; g 0.2 M; j: 0.1 M levulinic acid) a tendency to membrane localization is obvious (a, d, h are negative controls).