Research Article
Comparative Analysis of the Methanogen Diversity in Horse and Pony by Using mcrA Gene and Archaeal 16S rRNA Gene Clone Libraries
Table 1
PCR primers used in real-time PCR assays and in clone library analysis.
| Application | Target | Name (direction) | Sequence (5′→3′) | Annealing temperature (°C) | Number of cycles | Product size (bp) | Reference |
| Quantitative real-time PCR | Anaerobic fungi | q-pcr (f) | GAGGAAGTAAAAGTCGTAACAAGGTTTC |
60 |
40 |
120 |
[29] | q-pcr (r) | CAAATTCACAAAGGGTAGGATGATT | Methanogen | q-mcra (f) | TTCGGTGGATCDCARAGRGC |
60 |
40 |
141 |
[23] | q-mcra (r) | GBARGTCGWAWCCGTAGAATCC | Total bacteria | 1114 (f) | CGGCAACGAGCGCAACCC |
60 |
40 |
130 |
[29] | 1275 (r) | CCATTGTAGCAGGTG |
| 16S rRNA library | Methanogen | Met 86 (f) | GCTCAGTAACACGTGG |
53 |
30 |
1254 |
[30] | Met 1340 (r) | CGGTGTGTGCAAGGAG |
| mcrA library | Methanogen | mcrA(f) | GGTGGTGTMGGATTCACACARTAYGCWACAGC |
58 |
30 |
480 |
[31] | mcrA (r) | TTCATTGCRTAGTTWGGRTAGTT |
|
|
f: forward; r: reverse.
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