Research Article

Comparative Analysis of the Methanogen Diversity in Horse and Pony by Using mcrA Gene and Archaeal 16S rRNA Gene Clone Libraries

Table 3

The number of clones and similarity of the deduced amino acid sequences of the mcrA gene of each operational taxonomic unit (OTU) to cultured methanogens in horse and pony.

OTUsNearest known methanogen*Number of clones
HorsePonyTotal

Methanomicrobiales
OTU1Methanocorpusculum labreanum [NC_008942] (93)293362
OTU2Methanocorpusculum labreanum [NC_008942] (90)101323
OTU3Methanocorpusculum labreanum [NC_008942] (93)606
OTU4Methanocorpusculum labreanum [NC_008942] (94)112

Methanobacteriales
OTU5Methanobrevibacter smithii [DQ251046] (94)022
OTU6Methanobrevibacter gottschalkii [ACK56066] (100)101

Methanoplasmatales
OTU7Candidatus Methanomethylophilus alvus [KC412011] (80)112
OTU8Candidatus Methanomethylophilus alvus [KC412011] (92)101
OTU9Candidatus Methanomethylophilus alvus [KC412011] (83)101

Number in brackets and in parenthesis is accession number and similarity value (%), respectively.