Research Article

Comparative Analysis of the Methanogen Diversity in Horse and Pony by Using mcrA Gene and Archaeal 16S rRNA Gene Clone Libraries

Table 4

The number of clones and similarity of the archaeal 16S rRNA gene sequences of each operational taxonomic unit (OTU) to cultured methanogens in horse and pony.

OTUsNearest known methanogen*Number of clones
HorsePonyTotal

Methanomicrobiales
OTU1Methanocorpusculum labreanum [NR_074173] (96)323769

Methanosarcinales
OTU2Methanomicrococcus blatticola [AJ238002] (94)415

Methanobacteriales
OTU3Methanocorpusculum sinense [FR749947] (90)011
OTU4Methanobrevibacter  ruminantium [CP001719] (97)156
OTU5Methanobrevibacter smithii [NR_074235] (97)606
OTU6Methanobrevibacter  ruminantium [CP001719] (98)224
OTU7Methanobrevibacter  ruminantium [CP001719] (94)011

Methanoplasmatales
OTU8Candidatus Methanomethylophilus alvus [KC412010] (91)134
OTU9Candidatus Methanomethylophilus alvus [KC412010] (93)202
OTU10Candidatus Methanomethylophilus alvus [KC412010] (97)202

Number in brackets and in parenthesis is accession number and similarity value (%), respectively.