Comparative Analysis of the Methanogen Diversity in Horse and Pony by Using mcrA Gene and Archaeal 16S rRNA Gene Clone Libraries

Table 5

General information and diversity indices of the mcrA gene and archaeal 16S rRNA gene clone libraries recovered from microbial populations in the fecal contents of horse and pony.

Item	mcrA gene		16S rRNA gene
	Horse	Pony	Horse	Pony
Number of clones	50	50	50	50
Number of OTUs	8	5	8	7
Coverage (%)	37.5	86	75	57
Shannon-Wiener index (𝐻^′)	1.28	0.91	1.29	0.99
Evenness	0.33	0.23	0.33	0.25
Chao-1 species richness	18	7	12	13
LIBSHUFF analysis	Ns		𝑃<0.05

Ns: not significant.