Early Methanogenic Colonisation in the Faeces of Meishan and Yorkshire Piglets as Determined by Pyrosequencing Analysis

Table 1

Phylotype coverage and diversity estimation of the 16S rRNA gene libraries from faecal methanogens of Meishan and Yorkshire piglets¹.

Breed	Age (d)	OTUs	ACE	Chao	Shannon	Simpson	Coverage
Meishan	1	340.3	697.1	564.2	4.263	0.045	0.938
	3	235.8	484.0	398.3	3.551	0.085	0.958
	7	193.0	470.9	343.1	2.797	0.207	0.963
	14	179.8	502.4	353.9	2.505	0.279	0.963
Yorkshire	1	412.3	783.4	658.4	4.732	0.029	0.928
	3	427.0	791.1	689.4	4.789	0.028	0.924
	7	160.3	416.8	277.8	2.311	0.326	0.968
	14	126.0	434.9	272.0	1.764	0.419	0.972
SEM^2		137.7	250.95	214.0	1.297	0.168	0.023
Effect (P value)	Breed	0.184	0.409	0.307	0.664	0.185	0.236
	Age	0.000	0.048	0.001	0.000	0.000	0.001
	Breed × age	0.049	0.346	0.102	0.063	0.138	0.092

1The operational taxonomic units (OTUs) were defined with 3% dissimilarity. The coverage percentages, richness estimators (ACE and Chao), and diversity indices (Shannon and Simpson) were calculated.
2SEM: standard error of means, 𝑛=4.