Development of β-Lactamase as a Tool for Monitoring Conditional Gene Expression by a Tetracycline-Riboswitch in Methanosarcina acetivorans
Figure 3
tc-RS-regulated translation of bla mRNA. Left: scheme of tc-RS encoding blaN fusions inserted into the M. acetivorans chromosome; right: tc-RS variants analyzed in this study (tc-RS1 to tc-RS5); for simplicity, only the closing stem P1 and the adjacent RBS (in green) are shown and bases changed from the precursor construct are indicated in violet; mcrB P: methanoarchaeal mcrB promoter.
