Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna

Figure 4

Phylogenetic tree of archaeal 16S rRNA gene OTUs (97%) obtained from Cerrado freshwater sediments in the dry season (RIB-A D and RIB-B D sequences) and in the dry to rainy season transition period (RIB-A T and RIB-B T sequences). The number of sequences that are represented by a specific node of the tree is indicated in parenthesis. The phylogenetic tree was inferred by the maximum-likelihood method using the MEGA software, bootstrap value of 1,000, and Tamura-Nei nucleotide substitution model. The percentage of bootstrap resamplings that supports each topological element is represented near the line. Reference sequences from different phyla were used for comparison and the bacterial sequence of the uncultured Acidobacterium sp. JQ825222 was used as an outgroup. Bootstrap values below 50 were not shown and singletons were not included.