Sequence, Structure, and Binding Analysis of Cyclodextrinase (TK1770) from T. kodakarensis (KOD1) Using an In Silico Approach

Figure 1

Sequence alignment of Tk1770 CDase with sixteen CD hydrolyzing enzymes. The alignment of Tk1770 CDase with archeal and bacterial CD hydrolyzing enzymes was carried out with Clustal Omega through UGENE package. The novel N′-domain (CBM48) in archeal sequences is represented in red and the protruding region of CBM48 domain in green dotted line. The arrow shows the start of the TIM barrel domain (residues 204–584) and four conserved regions (I–IV) with another downstream conserved region V are represented in grey line below sequence. The catalytic triad is indicated through esterics. The HLH region of archeal sequences that is absent in all bacterial homologs is represented in blue dotted line.