Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization

Figure 3

Influence of temperature on the activity and influence of pH on the activity and stability of CDase-Tk. (a) Hydrolytic activity of CDase-Tk to pullulan, starch, and cyclodextrin. (b) Optimal temperature of CDase-Tk. (c) Optimal pH of CDase-Tk. Different buffers were used for the different pH solutions used in this assay. Sodium acetate was used for pHs 3.0, 4.0, and 5.0; MES buffer was used for pHs 5.0 to 7.5; HEPES buffer was used for pHs 8 and 8.5; glycine buffer was used for pHs 9.0 and 10.0. The concentrations of the buffers were 50 mM.

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