Characterization of Fatty Acids in Crenarchaeota by GC-MS and NMR

Figure 2

GC-MS analysis of fatty acids from cell extracts of S. solfataricus. Total Ion Chromatograms (TICs) of fatty acids are shown for S. solfataricus cell extracts (a) and lipid standard (b) as analyzed by GC-MS. Two lipids were positively identified from this analysis, matching gas chromatogram retention times and fragmentation patterns of known standards from the NIST database. GC peaks are labeled as follows: 1, hexadecanoic acid (C16:0); 2, hexadecenoic acid (C16:1); 3, octadecanoic acid (C18:0); 4, octadecenoic acid (C18:1); and 5, octadecadienoic acid (C18:2). The unlabeled peaks could not be positively identified but are believed to be due to instrument or solvent background based on NIST database searches.

(a)

(b)