Characterization of Fatty Acids in Crenarchaeota by GC-MS and NMR

Figure 3

GC-MS analysis of fatty acids from cell extracts of I. hospitalis and I. hospitalis-N. equitans coculture. Total Ion Chromatograms (TICs) of fatty acids present in I. hospitalis cell extracts (a), I. hospitalis-N. equitans coculturecell extraction (b), and lipid standard (c) as analyzed by GC-MS. Four lipids were positively identified from this analysis, matching retention times of known standards and fragmentation patterns in the NIST database. GC peaks are labeled as follows: 1, tetradecanoic acid (C14:0); 2, hexadecanoic acid (C16:0); 3, hexadecenoic acid (C16:1); 4, octadecanoic acid (C18:0); 5, octadecenoic acid (C18:1); and 6, octadecadienoic acid (C18:2).

(a)

(b)

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