Biochemical Characterisation of Phage Pseudomurein Endoisopeptidases PeiW and PeiP Using Synthetic Peptides

Figure 1

Comparison of the composition of pseudomurein and murein from cell walls. G, N-acetylglucosamine; T, N-acetyltalosaminuronic acid; M, N-acetylmuramic acid. Methanogen pseudomurein contains L-amino acids, β (1-3) bonds, and N-acetyltalosaminuronic acid in the glycan chain and several unusual isopeptide bonds. Glu can be replaced by Asp; Ala can be replaced by Thr or Ser, in some strains. Bacterial murein contains D- and L-amino acids and β (1-4) bonds and N-acetylmuramic acid in the glycan chain. The figure was adapted and modified from Hartmann and König [5] and Kandler and König [3].