Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and Base/Alternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine

Figure 1

Influence of DNA polymerase-B on the activity of EndoQ. (a) Denaturing gel showing inhibition of EndoQ by Pol-B and Pol-B plus PCNA with uracil. (b) Denaturing gel showing inhibition of EndoQ by Pol-B and Pol-B plus PCNA with hypoxanthine. The numbers above the gel lane indicate the hydrolysis time in minutes. (c) Scans of the gels shown in (a) and (b) indicating the amount of substrate remaining with time. In these experiments Pol-B exo⁻ (D215A) was used. All experiments were repeated at least four times and the inhibition patterns observed were highly reproducible. The data points shown in the scans have an error of <±10%.

(a) EndoQ/uracil

(b) EndoQ/hypoxanthine

(c) Scans of gels shown in (a) and (b)