Research Article

Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress

Figure 2

Analysis of the pH optimum and pH stability of Bcp1. H2O2 removal was measured using the ferrothiocyanate method in the presence of DTT as the electron donor at 37°C. (a) The optimum pH was measured in buffers within the range pH 5.5–7.5. (b) pH stability was determined at 37°C by incubating Bcp1 in different buffers, pH 5.5 (◆), pH 6.0 (■), pH 6.5 (▲), and pH 7.0 (●), for the following times: 30 min, 1 h, 2 h, 4 h, and 16 h.
(a)
(b)