Unusually High Archaeal Diversity in a Crystallizer Pond, Pomorie Salterns, Bulgaria, Revealed by Phylogenetic Analysis

Table 1

Frequencies of OTUs within the Archaea domain derived from the 16S rRNA sequences from а crystallizer pond from Pomorie saltern, Bulgaria. Novel sequences (more than 3% phylogenetic distance), their relative abundance, their closest phylogenetic neighbors, and similarity among them are underlined.

OTU	Accession number (seq. length, bp)	Number of clones	Cluster affiliation	Results of BLAST analysis: closest match, (sequence number), [identity], (isolation source)
PA-30	LN865035	20	Halobacteriaceae, Halanaeroarchaeum	Uncultured haloarchaeon clone DE09024B01, KF591560.1, 94%, Extreme Hypersaline Meromictic Lake
PA-73	LN865045	5	Halobacteriaceae, Halanaeroarchaeum	Uncultured euryarchaeote clone DSFBPENV12arc_1E, KC465594.1, 98%, hypersaline pools, Salton Sea
PA-105	LN865050	4	Halobacteriaceae, Halanaeroarchaeum	Uncultured archaeon clone Kasin-A1-C11, HE604442.1, 93%, Hypersaline Lake Kasin, Southern Russia
PA-21	LN865033	1	Halobacteriaceae, Halanaeroarchaeum	Halanaeroarchaeum sulfurireducens strain HSR2, CP008874.1, 96%
PA-2	LN865027	17	Halobacteriaceae, Halorubrum	Halorubrum ezzemoulense CECT 7099, NR_113217.1, 98%
PA-103	LN865049	4	Halobacteriaceae, Halorubrum	Halorubrum sp. s5a-3, JN196466.1, 98%, saltern Cabo de Gata, Spain
PA-53	LN865040	3	Halobacteriaceae, Halorubrum	Halorubrum californiense LV_12B39, LN649806.1, 99%
PA-52	LN865039	1	Halobacteriaceae, Halorubrum	Halorubrum orientale strain EJ-52, NR_042510.1, 94%
PA-3	LN865028	17	Halobacteriaceae, Halonotius	Uncultured archaeon clone TSHNAA23, HQ157628.1, 98%, Sfax salterns, Tunisia
PA-61	LN865043	1	Halobacteriaceae, Halonotius	Uncultured archaeon clone SFE1F061, CU467145.1, 98%, solar saltern, Tunisia
PA-8	LN865029	7	Halobacteriaceae, Halovenus	Uncultured archaeon 16S rRNA gene, clone ss_048, AJ969840.1 97%, Salt Spring, British Columbia, Canada
PA-51	LN865038	3	Halobacteriaceae, Natronomonas	Uncultured Natronomonas sp. clone 209ZA09, FN391194.1, 93%, Sfax salterns, Tunisia
PA-139	LN865052	1	Halobacteriaceae, Natronomonas	Uncultured haloarchaeon clone Ston16S367, DQ889328.1, 93%, solar salterns, Adriatic Sea
PA-11	LN865031	2	Halobacteriaceae, Salinigranum	Halobacteriaceae archaeon ZS-5, KJ689293.1, 99%, Zhoushan marine solar salter, China
PA-54	LN865041	1	Halobacteriaceae, Salinigranum	Uncultured archaeon clone 1, HE604940.1, 98%, Hypersaline Lake Kasin, Southern Russia
PA-79	LN865046	2	Halobacteriaceae, Halomicrobium	Uncultured archaeon clone Kasin-A1-A06, HE604415.1, 93%, Hypersaline Lake Kasin, Southern Russia
PA-63	LN865044	2	Halobacteriaceae, Halogeometricum	Halogeometricum rufum isolate LV_13S50, LN649947.1, 93%
PA-57	LN865042	2	Halobacteriaceae, Haloarchaeobius	Uncultured haloarchaeon clone XKL11, JN714414.1, 98%, salt lake, Xinjiang, China
PA-33	LN865036	2	Halobacteriaceae, Salinirubrum	Salinirubrum litoreum strain YJ-63-S1, KC918824.2, 99%
PA-87	LN865048	1	Halobacteriaceae, Haloarcula	Haloarcula japonica strain JCM7785, NR_116082.1, 99%
PA-17	LN865032	1	Halobacteriaceae, Haloquadratum	Uncultured archaeon clone Kasin-A3-D05, HE604599.1, 96%, Hypersaline Lake Kasin, Southern Russia
PA-145	LN865053	1	Halobacteriaceae, Halorhabdus	Uncultured Halorhabdus sp. clone SFH1C101, FN391257.1, 94%, solar saltern, Tunisia
PA-89	LN865030	7	Haloferaceae, Halobellus	Halobellus litoreus strain JCM 17118, NR_125476.1, 96%
PA-80	LN865047	1	Haloferaceae, Haloferax	Haloferax denitrificans strain JCM 8864, NR_113439.1, 90%
PA-26	LN865034	2	Unclassified	Uncultured euryarchaeote clone DSFBPENV12arc_7G, KC465577.1, 95%, hypersaline pools in the Salton Sea, US
PA-112	LN865051	1	Unclassified	Uncultured archaeon clone ss_014, AJ969886.1, 95%, Salt Spring, British Columbia, Canada