Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane

Figure 3

Phylogenetic tree and distribution of archaeal 16S rRNA genes retrieved from the sludge taken at various heights (0.3–4 m) of the full-scale UASB reactor for treating tetramethylammonium-rich wastewater. The 16S rRNA gene sequences were obtained through the DGGE-cloning approach, and the representative clones were shown in bold in the tree. The values in the square brackets indicated the numbers of clones that were similar to the representative phylotypes (>99% sequence similarity). The 16S rRNA gene sequence of Methanopyrus kandleri (NC003551) was used as an outgroup to root the tree. Bootstrap values of 50–75% and >75% were indicated with black circles and squares, respectively, at the nodes of the tree. The scale bar represented the estimated number of nucleotide changes per sequence position.