Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane

Table 1

Reads and diversity indices obtained from the high-throughput analysis of archaeal and bacterial 16S rRNA gene amplicons for sludge samples taken from tetramethylammonium-degrading CMSS and UASB reactors.

Sample ID	Reactor	Target	Height (cm)	Reads^1	Richness^2	Chao1^3	Shannon^4
UASB1a	UASB	Archaea	30	12,000	447	839	3.30
UASB2a	UASB	Archaea	100	12,000	703	1341	3.94
UASB3a	UASB	Archaea	200	12,000	710	1174	4.13
UASB4a	UASB	Archaea	300	12,000	641	1196	4.09
UASB5a	UASB	Archaea	400	12,000	282	500	2.04
UASB1a	UASB	Bacteria	30	20,000	218	270	5.02
UASB3a	UASB	Bacteria	200	20,000	249	266	5.88
UASB5a	UASB	Bacteria	400	20,000	299	384	4.37
CMJP	CMSS	Archaea	NA	12,000	250	367	2.75
CMJP	CMSS	Bacteria	NA	20,000	232	375	2.54

¹Number of qualified 16S rRNA sequence reads. ²Number of detected OTUs (97% similarity). ³Chao1 richness estimate; a higher number represents a higher degree of diversity. ⁴Shannon–Weiner index; a higher number represents a higher degree of diversity. NA: not applicable.