Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Figure 3

Effects of length of the flanking homologous region on transformation efficiency. SK-1 was transformed with 1 μg of a linear marker cassette attached to flanking regions (10–50 bp) of various lengths at both ends. The resulting colonies were counted. Open circles: regeneration in MBS; closed circles: direct plating (no regeneration). Error bars represent the standard deviation of three independent experiments.