Research Article

Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Figure 6

Growth curve of DNA photolyase-deficient strain after UV irradiation. Wt (SK-1) and Δphr (DP-1) cells were irradiated with UV light (1200 J/m2) and cultivated in XTU liquid medium under light or dark conditions for viability testing. (a) SK-1. (b) DP-1. Open symbols: light conditions; closed symbols: dark conditions; solid lines, without UV irradiation; dotted lines: with UV irradiation. Error bars represent the standard deviation of two independent experiments.
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