Comparative Genomic Analysis of Members of the Genera Methanosphaera and Methanobrevibacter Reveals Distinct Clades with Specific Potential Metabolic Functions

Figure 1

Single- and multilocus sequence analysis of Methanobrevibacter and Methanosphaera species. A maximum likelihood tree (left) of 16 Methanobrevibacter and three Methanosphaera genomes was inferred with 500 bootstraps with RAxML and visualized with Dendroscope. Phylogeny of Methanobrevibacter and Methanosphaera based on the 16S rRNA gene is shown on the right. The tree was resampled 500 times, and only bootstrap values ≥ 70% are shown. The 16S rRNA tree was rooted with five Methanobacterium sequences. The scale bar indicates 0.10 inferred nucleotide substitutions per position. Red-colored dots indicate the presence of mtaABC genes in the species/clade, green-colored dots indicate the presence of walB and walC gene homologues (potential utilization of ethanol), blue-colored dots indicate the presence of nitrogenase genes, and black-colored dots indicate the presence of carbon monoxide/acetyl CO-DH genes (see also Table S3 for details).