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Anatomy Research International
Volume 2012 (2012), Article ID 206238, 9 pages
http://dx.doi.org/10.1155/2012/206238
Review Article

The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens

1Laboratory of Biomedical Physics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
2Evolutionary Morphology of Vertebrates, Ghent University, K. L. Ledeganckstraat 35, 9000 Gent, Belgium

Received 28 April 2011; Accepted 12 August 2011

Academic Editor: Tuncay Peker

Copyright © 2012 Jan A. N. Buytaert et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions—orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)—is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.