Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells
Figure 4
Autofluorescence detected in freshly isolated testis cells using epifluorescent and confocal laser scanning microscopes. Testis cells were isolated from 1-wk-old piglet testes and examined under an epifluorescent microscope (a–c) equipped with filters of A (a), I3 (b), and N21 (c). Confocal laser scanning microscopy using an excitation laser of 405 nm, brightfield (d), or brightfield overlaid with acquired signals from spectrums of blue (440–490 nm, e), green (495–570 nm, f), yellow (575–620 nm, g), and red (625–780 nm, h). Scale bars, 50 μm.
