Evaluation of Hepatic Mitochondria and Hematological Parameters in Zidovudine-Treated B6C3F₁ Mice

Table 3

Effect of AZT (600 mg/kg/d) on the expression levels of genes in the liver of B6C3F₁ mice.

Gene description (gene symbol)	GenBank accession ID	FC	P
Apoptosis (18), 𝑃=0.039
Bcl2-associated X protein (Bax)	NM_007527	1.143	0.020
Bcl2 binding component 3 (Bbc3)	NM_133234	1.065	0.032
Bcl2/adenovirus E1B 19 k Da-interacting protein 1, NIP3 (Bnip3)	NM_009760	1.159	0.041
Lipid metabolism (23), 𝑃=0.046
Acyl-Coenzyme A synthetase long-chain family member 3 (Acsl3)	AK012088	1.166	0.001
Acyl-Coenzyme A dehydrogenase, long-chain (Acadl)	NM_007381	−1.159	0.025
Acyl-Coenzyme A dehydrogenase, short-chain (Acads)	NM_007383	−1.142	0.022
Acyl-Coenzyme A dehydrogenase, short/branched chain (Acadsb)	NM_025826	−1.225	0.011
Mitochondrial genes (13), 𝑃=NS
NADH ubiquinone dehydrogenase, subunit 1 (mt-Nd1)	AK018753	1.080	0.310
NADH ubiquinone dehydrogenase, subunit 2 (mt-Nd2)	AU018363	1.080	0.280
NADH ubiquinone dehydrogenase, subunit 3 (mt-Nd3)	J01420	1.021	0.724
NADH ubiquinone dehydrogenase, subunit 4 (mt-Nd4)	AB042809	1.018	0.827
NADH ubiquinone dehydrogenase, subunit 4L (mt-Nd4l)	AA109866	1.048	0.526
NADH ubiquinone dehydrogenase, subunit 5 (mt-Nd5)	AU018713	1.050	0.564
NADH ubiquinone dehydrogenase, subunit 6 (mt-Nd6)	AA435281	1.081	0.144
Cytochrome b (mt-Cytb)	AU018811	1.013	0.861
Cytochrome c oxidase, subunit I (mt-Co1)	AU018394	1.014	0.829
Cytochrome c oxidase, subunit II (mt-Co2)	AU019143	1.054	0.449
Cytochrome c oxidase, subunit III (mt-Co3)	AB042432	1.018	0.765
ATP synthase, subunit 6 (mt-ATP6)	AF093677	1.008	0.906
ATP synthase, subunit 8 (mt-ATP8)	J01420	1.067	0.464

The bold numbers in parentheses indicate number of genes evaluated for the molecular function. P values in bold represent effect of AZT on the molecular function, which is a combined effect of AZT-related changes in expression levels of number of genes evaluated for the molecular function. FC indicates a relative fold change in mice treated with 600 mg/kg/d AZT compared to vehicle-treated control mice. Negative values of fold changes indicate down-regulation of genes. Mitochondrial genes important in energy production, for which 600 mg/kg/d AZT did not alter transcription, are also presented in the table. Sex-related differences in the expression levels of mitochondria-related genes were not observed in liver of AZT-treated mice. NS: Not significant.