Table 3: Effect of AZT (600 mg/kg/d) on the expression levels of genes in the liver of B6C3F1 mice.

Gene description (gene symbol) GenBank accession ID FC P Apoptosis (18), Bcl2-associated X protein (Bax) NM_007527 1.143 0.020 Bcl2 binding component 3 (Bbc3) NM_133234 1.065 0.032 Bcl2/adenovirus E1B 19 k Da-interacting protein 1, NIP3 (Bnip3) NM_009760 1.159 0.041 Lipid metabolism (23), Acyl-Coenzyme A synthetase long-chain family member 3 (Acsl3) AK012088 1.166 0.001 Acyl-Coenzyme A dehydrogenase, long-chain (Acadl) NM_007381 −1.159 0.025 Acyl-Coenzyme A dehydrogenase, short-chain (Acads) NM_007383 −1.142 0.022 Acyl-Coenzyme A dehydrogenase, short/branched chain (Acadsb) NM_025826 −1.225 0.011 Mitochondrial genes (13),  NADH ubiquinone dehydrogenase, subunit 1 (mt-Nd1) AK018753 1.080 0.310 NADH ubiquinone dehydrogenase, subunit 2 (mt-Nd2) AU018363 1.080 0.280 NADH ubiquinone dehydrogenase, subunit 3 (mt-Nd3) J01420 1.021 0.724 NADH ubiquinone dehydrogenase, subunit 4 (mt-Nd4) AB042809 1.018 0.827 NADH ubiquinone dehydrogenase, subunit 4L (mt-Nd4l) AA109866 1.048 0.526 NADH ubiquinone dehydrogenase, subunit 5 (mt-Nd5) AU018713 1.050 0.564 NADH ubiquinone dehydrogenase, subunit 6 (mt-Nd6) AA435281 1.081 0.144 Cytochrome b (mt-Cytb) AU018811 1.013 0.861 Cytochrome c oxidase, subunit I (mt-Co1) AU018394 1.014 0.829 Cytochrome c oxidase, subunit II (mt-Co2) AU019143 1.054 0.449 Cytochrome c oxidase, subunit III (mt-Co3) AB042432 1.018 0.765 ATP synthase, subunit 6 (mt-ATP6) AF093677 1.008 0.906 ATP synthase, subunit 8 (mt-ATP8) J01420 1.067 0.464

The bold numbers in parentheses indicate number of genes evaluated for the molecular function. P values in bold represent effect of AZT on the molecular function, which is a combined effect of AZT-related changes in expression levels of number of genes evaluated for the molecular function. FC indicates a relative fold change in mice treated with 600 mg/kg/d AZT compared to vehicle-treated control mice. Negative values of fold changes indicate down-regulation of genes. Mitochondrial genes important in energy production, for which 600 mg/kg/d AZT did not alter transcription, are also presented in the table. Sex-related differences in the expression levels of mitochondria-related genes were not observed in liver of AZT-treated mice. NS: Not significant.