Table 3: Effect of AZT (600 mg/kg/d) on the expression levels of genes in the liver of B6C3F1 mice.

Gene description (gene symbol)GenBank accession IDFCP

Apoptosis (18),

Bcl2-associated X protein (Bax)NM_0075271.1430.020
Bcl2 binding component 3 (Bbc3)NM_1332341.0650.032
Bcl2/adenovirus E1B 19 k Da-interacting protein 1, NIP3 (Bnip3)NM_0097601.1590.041

Lipid metabolism (23),

Acyl-Coenzyme A synthetase long-chain family member 3 (Acsl3)AK0120881.1660.001
Acyl-Coenzyme A dehydrogenase, long-chain (Acadl)NM_007381−1.1590.025
Acyl-Coenzyme A dehydrogenase, short-chain (Acads)NM_007383−1.1420.022
Acyl-Coenzyme A dehydrogenase, short/branched chain (Acadsb)NM_025826−1.2250.011

Mitochondrial genes (13), 

NADH ubiquinone dehydrogenase, subunit 1 (mt-Nd1)AK0187531.0800.310
NADH ubiquinone dehydrogenase, subunit 2 (mt-Nd2)AU0183631.0800.280
NADH ubiquinone dehydrogenase, subunit 3 (mt-Nd3)J014201.0210.724
NADH ubiquinone dehydrogenase, subunit 4 (mt-Nd4)AB0428091.0180.827
NADH ubiquinone dehydrogenase, subunit 4L (mt-Nd4l)AA1098661.0480.526
NADH ubiquinone dehydrogenase, subunit 5 (mt-Nd5)AU0187131.0500.564
NADH ubiquinone dehydrogenase, subunit 6 (mt-Nd6)AA4352811.0810.144
Cytochrome b (mt-Cytb)AU0188111.0130.861
Cytochrome c oxidase, subunit I (mt-Co1)AU0183941.0140.829
Cytochrome c oxidase, subunit II (mt-Co2) AU0191431.0540.449
Cytochrome c oxidase, subunit III (mt-Co3)AB0424321.0180.765
ATP synthase, subunit 6 (mt-ATP6)AF0936771.0080.906
ATP synthase, subunit 8 (mt-ATP8)J014201.0670.464

The bold numbers in parentheses indicate number of genes evaluated for the molecular function. P values in bold represent effect of AZT on the molecular function, which is a combined effect of AZT-related changes in expression levels of number of genes evaluated for the molecular function. FC indicates a relative fold change in mice treated with 600 mg/kg/d AZT compared to vehicle-treated control mice. Negative values of fold changes indicate down-regulation of genes. Mitochondrial genes important in energy production, for which 600 mg/kg/d AZT did not alter transcription, are also presented in the table. Sex-related differences in the expression levels of mitochondria-related genes were not observed in liver of AZT-treated mice. NS: Not significant.