735472.fig.002a
(a)
735472.fig.002b
(b)
Figure 2: In vitro gene transduction efficiency in equine chondrocytes and synovial cells transfected by adenoviral (Ad), recombinant adeno-associated viral (rAAV2), or self-complementary AAV (scAAV2) vectors carrying green fluorescent protein (GFP) genes at three different dosages; low, medium, and high dosage levels for Ad-GFP were 1e4, 1e5, and 1e6 particles per cell, and low, medium, and high dosage levels for rAAV2/scAAV2 were 1e6, 1e7, and 1e8 DRP per cell. (a) Representative fluorescent microscopic images (approximately 90% cell confluence at day 7, and 100% cell confluence at day 14 and 28) and (b) the computed %transduction of equine chondrocytes and synovial cells. *Significantly greater gene transduction in same dosage level compared to Ad and rAAV2 vectors. +Significantly greater gene transduction in same dosage level compared to Ad vectors. #Significantly greater gene transduction in same dosage level compared to rAAV2 vectors.