943156.fig.003a
(a)
943156.fig.003b
(b)
943156.fig.003c
(c)
943156.fig.003d
(d)
943156.fig.003e
(e)
Figure 3: Flow cytometric analysis of a cell population positive for membrane-bound IL-15 (R1 region). Density plot had shown different population of cells in synovial fluid (a). Fluorescence intensity in R1 region is represented by white histograms, using a specific antibody MAB 2471(b); specific antibodies to detect CD3 (c) or CD8 (d) and gray histograms refer to the background staining. Acid treatment with acid buffer (pH 3.0) before incubation with MAB 2471 produced a slight decrease in fluorescence intensity (bold gray line) in comparison to incubation with MAB 2471 in PBS (dotted line) (e).