New Aspects in the Differential Diagnosis and Therapy of Bladder Pain Syndrome/Interstitial Cystitis
Table 1
Antibodies used in indirect confocal immunofluorescence.
(a) Primary antibodies
Primary antibodies
Host
Source
Order no.
Dilution
M2, muscarinic receptor
rabbit
[1]
AS-3721S
1 : 1000
M3, muscarinic receptor
rabbit
[1]
AS-3741S
1 : 1000
P2X1, purinergic receptor
rabbit
[2]
ab10248
1 : 1000
P2X2, purinergic receptor
rabbit
[2]
ab10266
1 : 1000
P2X3, purinergic receptor
rabbit
[2]
ab10269
1 : 1000
H1, human histamine receptor 1
rabbit
[3]
H1R12-A
1 : 250
H2, human histamine receptor 2
rabbit
[3]
H2R22-A
1 : 250
HCG (beta-1 epitope)
mouse, IgG1
[5]
MCA19
1 : 500
HCG (beta-2 epitope)
mouse, IgG1
[5]
MCA329
1 : 20
alpha-smooth muscle cell actin
mouse, IgG2a
[4]
A2547
1 : 2000
(b) Secondary antibodies
Secondary antibodies
Source
Order no.
Dilution
Alexa Fluor 488 goat antimouse IgG2a
invitrogen
A-21131
1 : 500
Alexa Fluor 555 goat antirabbit
invitrogen
A-21428
1 : 500
Alexa Fluor 555 goat antimouse
invitrogen
A-21127
1 : 500
[1] Research & Diagnostic Antibodies, North Las Vegas, USA. [2] Abcam Inc., Cambridge, USA. [3] Alpha Diagnostic Intl. Inc., San Antonio, USA. [4] Sigma-Aldrich Chemie GmbH, Steinheim, Germany. [5] AbD Serotec, MorphoSys AG, Martinsried/Planegg, Germany.
