Research Article
Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?
Table 1
Clinical and laboratory data of studied groups (mean ± SD).
| Variables | Varicocele group () | Fertile group () | value |
| Sperm count (mil/mL) | 26.2 ± 2.7 | 74.2 ± 19.2 | 0.005 | Motility A + B (%) | 27.8 ± 16.5 | 62.5 ± 11.9 | 0.02 | Normal forms (%) | 59.8 ± 13.6 | 71.03 ± 8.2 | 0.02 | Sperm DFI (%) by acridine orange | 32.4 ± 7.4 | 18.2 ± 4.8 | 0.003 | GMFI of sperm DNA chromatin condensation by flow cytometry (%) | 25.4 ± 8.8 | 12.8 ± 2.2 | 0.005 | Mean age (years) | 31 ± 8.2 | 30.2 ± 2 | NS |
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Significant . GMFI: the geometric mean fluorescence intensity. DFI: DNA fragmentation index.
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